Original Investigation

Prevalence of Illicit Drug Detection in 5 US Cities Among Out-of-Treatment People Who Inject Drugs

JAMA Network Open 10.1001/jamanetworkopen.2025.55882

February 05, 2026 at 11:00 AM EST

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What is the prevalence of illicit drug detection among people who inject drugs who are not engaged in medical care across 5 US cities?In this cross-sectional study of 444 people who inject drugs, fentanyl was detected among 93%, xylazine among 53%, polysubstance drugs among 95%, and amphetamine among 67%; unhoused and recently incarcerated individuals had higher prevalence of cocaine and stimulant detection, respectively. Amphetamine prevalence increased over time in Washington, DC, and Philadelphia had the highest prevalence of xylazine detection.The findings suggest localized, real-time drug surveillance for out-of-treatment individuals who inject drugs, often missed by traditional public health systems, is needed.

Corresponding Author: Nabila El-Bassel, PhD, School of Social Work, Columbia University, 1255 Amsterdam Ave, New York, NY 10027 (ne5@columbia.edu).

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